DNAzyme Nanoparticles for the Cleavage of RNA


Site-selective RNA splicing nanozyme for cleaving and ligating RNA.

Key Benefits

  • Can cleave virtually any RNA stem loop.
  • Removes a 19 nucleotide segment from a 67 nucleotide RNA loop with up to 66% efficiency.

Technical Summary

Modulating RNA can be useful for studying RNA biology, gene knockdown, and regulating RNA splicing. Scientists at Emory University have developed a new DNAzyme nanoparticle for cleaving and ligating RNA. This technology encompasses a composite nanoparticle designed to cleave and ligate RNA targets. The composite NP is comprised of 4 pieces: a gold nanoparticle, two 10-23 DNAzymes that cut pre-mRNA at a certain purine/pyrimidine junction, and an RNA ligase that pastes RNA fragments back together.

Developmental Stage

Technology is available for license.

Publication: Petree, J. R. et al. (2018). ACS Chem. Biol, 13(1), 215-224.

Patent Information

App Type Country Serial No. Patent No. File Date Issued Date Patent Status
Utility (parent) United States 16/180,786 11,136,595 11/5/2018 10/5/2021 Issued
Tech ID: 18022
Published: 9/5/2019