BG505 SOSIP Vaccine Elicited Neutralizing Monoclonal Antibodies for HIV

Application

ELISA
Western Blot
Immunohistochemistry

Specficity

HIV-1 BG505 Env protein (clone name: YRK01, YRK02).

Subtype

Monoclonal antibody.

Immunogen

HIV-1 BG505 SOSIP Env trimer.

Technical Summary

HIV-1 envelope glycoprotein (Env) is a membrane protein complex involved in receptor recognition and viral fusion with CD4+ T cells that allows the virus to enter cells. Emory researchers have identified two clonally related monoclonal antibodies that were recovered from a BG505 SOSIP immunized rhesus macaque via PCR and cloning of the immunoglobulin heavy and light chain variable domain gene segments from single antigen specific B cells. The monoclonal antibodies potently neutralize the autologous BG505 envelope pseudovirus (a virus that infects susceptible cells, but only replicates once). This can be used as a research tool to generate neutralizing antibodies against HIV.

Publication: Ward and Wilson, Trends in Biochemical Sciences, 2015 Feb;40(2):101-7