Rabbit Polyclonal Antipike Antibody
Application
- Immunoblotting
- Immunoprecipitation
- Immunofluorescence
Specificity
Rabbit Polyclonal Anti-PIKE Antibody
Subtype
Anti-Rabbit
Technical Summary
PIKE-activating Akt (PIKE-A), an isoform of PIKE GTPase that enhances phosphatidyl- inositol 3-kinase (PI3-kinase) activity. PIKE will specifically binds to active Akt but not PI3-kinase. PIKE-A stimulates Akt activity in a GTP-dependent manner and promotes invasive- ness of cancer cell lines. PIKE-A is amplified in a variety of human cancers and that amplified PIKE-A directly stimulates Akt and inhibits apoptosis compared to cells with normal PIKE-A copy number. Overexpression of PIKE-A wild-type but not dominant-negative mutant stimulates Akt activity and prevents apoptosis. Moreover, knockdown of PIKE-A diminishes Akt activity and increases apoptosis. It is suggested that PIKE-A amplification may contribute to cancer cell survival and progression by inhibiting apoptosis through up regulating Akt.
Publications
Proc. Natl. Acad. Sci. 101: 18, 6993-6998 (2004)
J. Biol. Chem. 279: 16, 16441-16451 (2004).
J. Cell Sci. 117: Pt. 2: 155-161 (2004).
Patent Information
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