CIITA Deficient Human B Cell Line

Application

Human B cell line deficient in class II transactivator (CIITA).

Key Benefits

Deficient in CIITA.
Useful for expression of tranfected MHC-II genes and alleles.

Technical Summary

The class II transactivator (CIITA) is essential for the expression of major histocompatibility complex class II (MHC-II) genes. However, the role of CIITA in gene regulation outside of MHC-II biology is not fully understood. Researchers at Emory University have developed a CIITA deficient human B cell line derived from Raji, a human Burkitt’s lymphoma cell line. In addition to being able to study CIITA or CIITA independent effects on MHC class II regulation, these cells can serve as a host cell for the expression of transfected MHC-II genes and alleles and accessory molecules. These cells could be used to probe antigen presentation of vaccine candidates or diagnostic markers associated with MHC-II.