Technology Listings


Library of Lipase-Based Biocatalysts Derived from CALB

Application

A lipase-based platform technology that can be used in the production of fine chemicals, detergents, and pharmaceuticals.

Key Benefits
  • Enhanced thermostability, activity, and enantioselectivity of enantiopure compounds including bulky substrates.
  • Can be used at a concentration 100x less than that of other enzymes.
Technical Summary

Lipases are a group of specialized enzymes that are also important biocatalysts for the chiral synthesis of the active ingredients of pharmaceuticals and specialty chemicals. Among the most commonly used biocatalysts is lipase B from Candida Antarctica (CALB). The broad substrate specificity of CALB accompanied by its ability to function in both aqueous and organic reactions make it a versatile tool for the kinetic resolution and polymerization of esters.

Emory University researchers have generated a library of CALB variants that can be used to optimize the enantioselective esterification of select laboratory and commercial compounds including ibuprofen. By creating circular permutants of CALB, Dr. Lutz and colleagues have generated a library of lipases with catalytic activity superior to wild type CALB. Importantly, the inherent enantioselectivity of CALB is not disrupted. Therefore, these novel CALB variants can be used to maximize the efficiency of a wide range of enzymatic reactions including the production of fine chemicals, detergents and pharmaceuticals.

Developmental Stage
  • Enzyme variants have been characterized and optimal ones have been selected.
  • Proof of principle has been established using select CALB variants.
Patent Information
App Type Country Serial No. Patent No. File Date Issued Date Expire Date
Continuation in Part United States 11/776,281 8,034,579 2/10/2006 10/11/2011 11/10/2027
Nationalized PCT - Foreign EP 06734704.7 1851324 2/10/2006 10/24/2012 2/10/2026
EP Registered Country United Kingdom 06734704.7 1851324 2/10/2006 10/24/2012 2/10/2026
EP Registered Country Germany 06734704.7 1851324 2/10/2006 10/24/2012 2/10/2026
EP Registered Country France 06734704.7 1851324 2/10/2006 10/24/2012 2/10/2026
Tech ID: 05031
Published: 2/24/2009
Category
Non-Therapeutics

Contact
Cliff Michaels
Assistant Director
Emory University
404-727-3890
ccmicha@emory.edu

Inventor(s)
Stefan Lutz
Zhen Qian

Keywords
Chemistry
Enzyme/Protein