- Western blot
Huntingtin Associated Protein 1A and B isoforms. No detectable cross reactivity to other proteins by Western blot.
Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by an expanded trinucleotide repeat in the IT15 gene. Huntington Associated Protein 1 (Hap1) was initially identified as a protein that interacts with huntingtin, the protein encoded by the gene responsible for the development of HD.
These antibodies can be used to specifically detect Hap-1A and B by immunoblotting or immunocytochemistry. They have been shown to detect Hap-1A and B from mouse, rat, and primate brain tissue. These antibodies can also be used for immunoprecipitation of Hap-1A and B from rat brain tissue.
Chan EY, et al., Hum Mol Genet. 2002 Apr 15; 11(8):945-59
Gutekunst CA, et al., J Histochem Cytochem. 2003 Jun; 51(6):841-52
Torre ER, et al., J Neurosci Methods. 2003 May 30; 125(1-2):27-32
Tang TS, et al., Neuron. 2003 Jul 17; 39(2):227-39