An antibody to detect mammalian PME-1 that can be used for immunoblotting and immunoflorescence and possibly histochemistry
Dr. Pallas has developed a polyclonal antibody to PME-1, a protein methylesterase for which PP2A (protein phosphatase 2A) is a substrate. PP2A is a highly conserved serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and other cellular events. In accordance with these diverse roles, PP2A has been found localized to the nucleus, cytoplasm, cytoskeleton, and membranes. This antibody detects PME-1 in cell lysates and immunoprecipitates and in fixed cells but does not immunoprecipitate native protein. It should be useful for investigating the role of PP2A methylation in cancers, Alzheimer's Disease, and other diseases in which dysregulation of PP2A has been implicated.