Can be used in assays to search for drugs that modulate protein phosphatase 2A (PP2A) methylesterase activity or to make recombinant protein to be used in assays. Such derivatives have potential application as anti-cancer and Alzheimer's treatment drugs.
Protein phosphatase 2A (PP2A) is involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events. Therefore, it is not surprising that PP2A is highly regulated. This invention describes the cloning of a modulating enzyme for human PP2A, named Protein Phosphatase Methylesterase-1 (PME-1). It provides the nucleotide sequences encoding PME-1 and the deduced amino acid sequence. In addition, this invention provides methods for producing recombinant PME-1 polypeptide, which can then be used for in vitro assays to identify inhibitors or activators of PME-1 activity. Such inhibitor or activator drugs are likely to modulate cell cycle, apoptosis, and tau hyperphosphorylation and may be useful in treating diseases such as cancer and Alzheimer's Disease.