Technology Listings


Shotgun Glycomics: A Microarray Strategy for Functional Glycomics

Application

Microarray based strategy for functional analyses of complex cellular glycomes.

Key Benefits
  • Identifies functionally relevant glycans.
  • Allows focused structural analyses of biologically important glycans.
  • New approach to label glycosphingolipids that retains their protein binding.
Technical Summary

Glycans mediate crucial cell-cell, cell-protein and cell-lipid interactions. Recognition and binding of glycans by glycan binding proteins (GBPs) is central to a variety of biological processes, such as movement and trafficking of white blood cells in inflammation and host pathogen recognition. In addition, many glycans are bound by antibodies and this is important in host defense as well as autoimmune disorders. Identifying functional glycans as ligands for GBPs, as well as antigenic glycans, and characterizing the highly complex cellular glycomes remains extremely challenging. The technology developed at Emory can be used to analyze glycans released from glycoproteins and cellular glycosphingolipids (GSLs) in a shotgun format. These isolated and purified glycans can then be used for identification of GBPs, and thereby define functionally relevant glycans. This strategy allows focused structural analyses on biologically important glycans, and will be especially useful in developing glycan-based therapeutics and diagnostics.

Researchers at Emory University have developed a novel shotgun glycomics strategy that allows identification of functional glycans derivatized from GSLs as ligands for GBPs. Derivatization of glycans from GSLs for functional glycomics is complicated since enzymatic release of glycans from GSLs might compromise their GBP binding. This new approach fluorescently labels GSLs that permits easy derivatization, quantification, and separation by HPLC and immobilization to glass slides to generate GSL shotgun microarrays. Probing of these microarrays by GBPs and antibodies identifies functional or antigens GSLs, which can then be characterized by mass spectrometry. Glycoprotein derived glycans could also potentially be analyzed using this technique.

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Developmental Stage

Anti-glycolipid antibodies have been identified in sera from patients with Lyme disease, and GSL microarrays and tagged glycan libraries (TGLs) have been prepared from human erythrocytes and PC3 cells.

Patent Information
App Type Country Serial No. Patent No. File Date Issued Date Expire Date
PCT PCT PCT/US2011/061765   11/22/2011   5/23/2013
Nationalized PCT - Foreign EP 11843357.2   11/22/2011    
Nationalized PCT - United States United States 13/885,916 11/22/2011    
Tech ID: 11034
Published: 4/10/2012
Category
Research Tools

Contact
Justin Burns
Licensing Associate
Emory University
justin.burns@emory.edu

Inventor(s)
Richard Cummings
David Smith
Xuezheng Song

Keywords
Assay
Chemistry