Polyclonal antibody to the PP2A pan-B' subunit
This antibody is useful in studying the B' family of PP2A subunits. The antibody was generated against a sequence highly conserved in all known PP2A B' subunits and is termed AR-1. AR-1 recognized known B' subunits that migrated at 56 and 74 kDa, as well as three unknown proteins that migrated at polyclonal antibody to a PP2A B' subunit peptide. This antibody works in Western blotting, but not for immunoprecipitation. The affinity-purified form is cleaner but both work well. This antibody cross-reacts with striatin and SG2NA due to the homologous peptide sequence and will immunoprecipitate striatin and SG2NA in the presence of 20% Triton. In addition to Western blotting, this antibody could perhaps be used for immunofluorescence or histochemistry and does not appear to immunoprecipitate native B' subunit.
J. Biol. Chem. Moreno et al. 2000; 275 (8): 5257-63.
J. Biol. Chem. Shern et al. 2003; 278 (42): 40829-36.