Technology Listings


Protein Phosphatase 2A Regulatory b' Subunit Polyclonal Antibody (AR1)

Applications
  • Immunohistochemistry
  • Immunofluorescence
  • Immunoblotting
Specificity

Polyclonal antibody to the PP2A pan-B' subunit

Subtype

Anti-rabbit

Technical Summary

This antibody is useful in studying the B' family of PP2A subunits. The antibody was generated against a sequence highly conserved in all known PP2A B' subunits and is termed AR-1. AR-1 recognized known B' subunits that migrated at 56 and 74 kDa, as well as three unknown proteins that migrated at polyclonal antibody to a PP2A B' subunit peptide. This antibody works in Western blotting, but not for immunoprecipitation. The affinity-purified form is cleaner but both work well. This antibody cross-reacts with striatin and SG2NA due to the homologous peptide sequence and will immunoprecipitate striatin and SG2NA in the presence of 20% Triton. In addition to Western blotting, this antibody could perhaps be used for immunofluorescence or histochemistry and does not appear to immunoprecipitate native B' subunit.

Publications

J. Biol. Chem. Moreno et al. 2000; 275 (8): 5257-63.
J. Biol. Chem. Shern et al. 2003; 278 (42): 40829-36.

Patent Information
Tech ID: 01093
Published: 2/24/2009
Category
Research Tools

Contact
Rajsekhar Guddneppanavar
Licensing Associate
Emory University
RGUDDNE@emory.edu

Inventor(s)
David Pallas

Keywords
Antibody