- Western blot
Peptide sequences conserved within several human 14-3-3 protein subspecies
Two rabbit sera identified as p81 and Q97 were made against synthetically synthesized peptides of 14-3-3 protein. The two antibodies are more specifically a beta-zeta 14-3-3 polyclonal antibody (p81) and a zeta-gamma ETA 14-3-3 polyclonal antibody (Q97).
Polyclonal rabbit serum was raised against the pep-tide ELVQKAKLAEQA synthesized on a polylysine core (MAP resin). The identical sequence is found in several 14-3-3 family members, including the zeta form, and less related versions are found in many other family members, including the epsilon form.
Publication: Pallas et al. Science. 265:535-537. (1994)