Cell-free assay system useful for discovering inhibitors of Nox enzymes. These inhibitors may be useful as drugs for cancer, atherosclerosis, hypertension, and inflammatory diseases such as arthritis, inflammatory bowel disease, etc.
Nox enzymes play an important role in mitogenic cell growth and angiogenesis, and their aberrant regulation is implicated in cancer and atherosclerosis. The Nox family consists of 7 unique gene products in humans, but regulatory proteins for Nox enzymes have not been previously described. This invention provides the following: nucleotide sequences encoding for proteins that regulate the activity of Nox enzymes, amino acid sequences of these regulatory proteins, vectors containing these nucleotide sequences, methods for transfecting cells with vectors that produce these regulatory proteins, and antibodies to these proteins that are useful for detecting and measuring levels of these proteins. These regulatory proteins provide a key technology needed for development of assays that reconstitute reactive oxygen generation by Nox enzymes, and hence are essential for the development for example of high throughput assays for inhibitors of reactive oxygen generation.