Hypoxia/Hypoxia-inducible factor 1 (HIF-1) inducible luciferase/LacZ reporter cell lines.
Expression of luciferase and beta-galactosidase in response to hypoxia treatment.
Emory University researcher, Erwin Van Meir, has made HIF/hypoxia reporter cell lines by stably transfecting the cells with pBIGL-V6R-HRE-Luc/LacZ reporter construct. The cell lines can be used to monitor transcriptional activity of the reporter in response to hypoxia or HIF transcriptional activity. The reporter activates both luciferase and b-galactosidase expression.
- LN229 human glioblastoma : pBIGL-V6R-HRE-Luc/LacZ
- Mel290 human uveal melanoma : pBIGL-V6R-HRE-Luc/LacZ
- B16LS9 mouse melanoma : pBIGL-V6R-HRE-Luc/LacZ
- 9L rat glioma : pBIGL-V6R-HRE-Luc/LacZ
Cell lines are developed and available for license.