Technology Listings


EU172 – Polyclonal Antibody against Human DNA Methyltransferase 3-like (DNMT3L)

Application

Western Blot

Specificity

Human Dnmt3L (full-length)

Subtype

Rabbit polyclonal

Immunogen

Native Protein

Technical Summary

DNA methyltransferase (DNMT) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3. The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3L does not contain amino acids necessary for methyltransferase activity, therefore it likely does not function as a DNA methyltransferase. It is instead believed to stimulate de novo methylation by DNMT3s. The antibody available was purified from rabbit using highly purified native protein as antigen. The dilution of the serum is 1:500 and the dilution of the purified antibodies is 1:5000. Reactivity was tested for both the serum and purified antibodies via dot blot using corresponding antigens.

Publication: Chang et al. Nat Commun. 2011 2:533.

Patent Information
Tech ID: 10008
Published: 1/28/2013
Category
Research Tools

Contact
Rajsekhar Guddneppanavar
Licensing Associate
Emory University
RGUDDNE@emory.edu

Inventor(s)
Xiaodong Cheng
Xing Zhang

Keywords
Antibody