Mouse Dnmt3b N-terminal domain
DNA methyltransferase (DNMT) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3. The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein. DNMT3B is believed to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. The antibody available was purified from rabbit using highly purified native protein as antigen. The dilution of the serum is 1:1000 and the dilution of the purified antibodies is 1:5000. Reactivity was tested for both the serum and purified antibodies via dot blot using corresponding antigens. This antibody against Dnmt3b does not cross react with Dnmt3a.
Publication: Chang et al. Nat Commun. 2011 2:533.