Technology Listings

Counterselectable Vector for Gram+ Bacteria


Counterselectable system for use in Gram+ bacteria.

Key Benefits
  • Useful for developing genetic systems in Gram+ bacteria.
  • Versatile design allows shuttling between Gram+ and Gram- bacteria.
Technical Summary

This technology is a counterselectable system for use in Gram+ (G+) bacteria. A plasmid vector has been constructed that can be selected against G+ bacteria by adding streptomycin to the medium. The rpsL streptococcal gene was cloned into a vector and allows selection against the plasmid and thus selection for integration of any homologous region that has been cloned into this vector.

This vector highly versatile and is useful for the genetic manipulation of G+ bacteria, including S.pyogenes, S. pneumonia, Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes, B.subtilis,and Lactococcus lactis. The vector is useful for designing an altered form of a gene and to replace a resident gene, or to add a new gene to the chromosome of one of these bacteria. It can be used to produce new products in one the organisms or to engineer a vaccine strain of one of the pathogens.

Patent Information
Tech ID: 97059
Published: 4/29/2009
Research Tools

Cliff Michaels
Assistant Director
Emory University

June Scott

Microbiology/Infectious Diseases