Counterselectable system for use in Gram+ bacteria.
- Useful for developing genetic systems in Gram+ bacteria.
- Versatile design allows shuttling between Gram+ and Gram- bacteria.
This technology is a counterselectable system for use in Gram+ (G+) bacteria.
A plasmid vector has been constructed that can be selected against G+ bacteria
by adding streptomycin to the medium. The rpsL streptococcal gene was cloned
into a vector and allows selection against the plasmid and thus selection for
integration of any homologous region that has been cloned into this vector.
This vector highly versatile and is useful for the genetic manipulation of G+
bacteria, including S.pyogenes, S. pneumonia, Staphylococcus aureus,
Enterococcus faecalis, Listeria monocytogenes, B.subtilis,and
Lactococcus lactis. The vector is useful for designing an altered form
of a gene and to replace a resident gene, or to add a new gene to the chromosome
of one of these bacteria. It can be used to produce new products in one the
organisms or to engineer a vaccine strain of one of the