- Flow Cytometry
- Functional Assays
- Western Blot
CD44v6 (v6 variant-exon containing form of CD44)
Antibody binding is increased in the epithelium of inflamed intestinal tissue in mice and in humans with inflammatory bowel disease. CD44v6 is a variation of CD44 that is reported as a cell surface marker for various cancers and inflammation. Emory Researchers have created an antibody, GM35 (an interferon gamma-stimulated intestinal epithelial cell), to bind to a carbohydrate antigen on CD44v6.
Compared to currently available antibodies for these antigens, this new antibody has greater affinity and specificity. Due to its high affinity for a ligand implicated as a marker of inflammation and now with an increasingly recognized role in the clearance of inflammatory cells, the GM35 antibody has potential uses as a research, diagnostic, and possibly therapeutic tool.
Publication: Si-Tahar, M., S. Sitaraman, et al. (2001). Cell Physiology 280(3): C423-C432.