Monoclonal antibodies specific for thr PP2A catalytic subunit.
The inventor has developed an anti-PP2A C-terminal peptide monoclonal antibodies, specific for the PP2A C subunit, 1d6, 4b7, and 4e1. These antibodies almost exclusively recognize the PP2A catalytic subunit in its unmethylated state. The antibodies are inhibited by methylation of PP2A and are therefore useful for assaying the PP2A methylation state in a quantitative manner. While 4b7, 4e1 and 1d6 are all immunoreactive, 1d6 gives the strongest signal in immunoblotting but may be the least methylation-sensitive. All three antibodies also immunoprecipitate PP2A complexes. Because of the conservation of the immunogenic peptide these antibodies react with a variety of species, 1d6 in particular is known to react with PP2A from all species tested (from yeast to mammals) but the others react with a wide variety of PP2As as well .
Publication: Yu et al., Mol. Biol. Cell 2001; 12:185-199. Immunofluorescence Histochemistry