Technology Listings


Vectors for the Bacterial Expression of Human Deubiquitinating Enzymes

Application

Vectors that express human deubiquitinating enzymes including UCH-L1, UCH-L3, Bap1, isopeptidase T (both short and long isoforms), and USP14.

Technical Summary

These vectors use a pRSET vector backbone and express human deubiquitiating enzymes including UCH-L1, UCH-L3, Bap1, isopeptidase T (both short and long isoforms), and USP14. These enzymes are expressed as full length proteins in bacteria under the control of the lac promoter. They can be used to produce recombinant enzymes.

Publications

Hu M., et al. (2002) EMBO.J. 24:3747-3756
Johnston S.C., et al. (1997) EMBO.J. 16:3787-3796
Johnston S.C., et al. (1999) EMBO.J. 18:3877-3887
Mayer A.N. and Wilkinson K.D. (1989) Biochem. 28:166-172
Wilkinson K.D., et al. (1989) Science. 246:670-673
Wilkinson K.D., et al. (1995) Biochemistry 34:14535-14546
Wilkinson K.D., et al. (1999) J. Mol. Biol. 291:1067-1077
Wilkinson K.D., et al. (2001) EMBO. J. 20:5187-5196

Patent Information
Tech ID: 02037
Published: 3/15/2013
Category
Research Tools

Contact
Hyeon (Sean) Kim
Licensing Associate
Emory University
hkim70@emory.edu

Inventor(s)
Keith Wilkinson

Keywords
Microbiology/Infectious Diseases