- Peroxidase Modeling
Human, mouse VPO1; does not detect other peroxidases.
Mammalian heme-containing peroxidases catalyze the oxidation of a variety of substrates by H2O2, playing roles in innate immunity as well as synthesis of hormones and extracellular matrix. Of particular medical importance, these enzymes have been implicated in a number of diseases, including asthma, Alzheimer's disease, and inflammatory vascular disease. Dr. Cheng's group has identified VPO1, a novel heme-containing peroxidase that is primarily expressed in the cardiovascular system and exhibits constitutive peroxidase activity.
The VPO1 antibodies generated by Dr. Cheng specifically recognize mouse and human VPO1 by immunoblotting, without detecting other heme-containing peroxidase family members. They are capable of detecting endogenous VPO1 in mice. Full-length mouse and human VPO1 is available in the form of stably-expressing cell lines, which can provide protein through simple purification methods.
Publication: Cheng, G et al. Free Radic. Biol. Med., 2008. 45:1682-94