Protein expression on the tip of pili in gram-positive bacteria including
Lactococcus lactis. Applications include the development of live
bacteria-based vaccines that do not require refrigeration and are inexpensive to
produce; and other live bacteria-based therapeutics, utility for food
processing, probiotic applications and as biocatalysts.
- Technology platform for a new bacterial expression system that covalently
links a protein external to the bacterial surface.
- Expression system has been demonstrated in Lactococcus lactis.
- Multiple potential applications including vaccines, probiotics and
Lactococcus, Lactobacillus, and other gram-positive bacteria have a
variety of industrial applications in the medical, agricultural and food
processing fields. Emory investigators have developed a technology platform for
protein expression on the surface of gram-positive bacteria. A fusion
protein has been covalently linked to the tip of a pilus and has been
successfully expressed in L. lactis. This technology allows for the
expression of candidate antigens, enzymes and other proteins external to the
bacterial surface. One potential application includes producing an oral
non-invasive vaccine that is inexpensive to manufacture and does not require
refrigeration. The technology can also be used to present enzymes either as part
of a human or animal probiotic, as a biocatalyst, for bioremdiation, or to
improve animal feed.
- Genetic engineering has allowed a successful covalent linkage of a foreign
protein to the tip of a pilus in L. lactis.
- Experiments are underway to produce and test biocatalysts and vaccines
using the expression system.