- Testing of therapeutics that seek to inhibit consequences of T
cell-mediated immune responses.
- Identification of chemicals with weak contact sensitizing activity.
The keratinocytes of the mouse model constitutively express both the
costimulatory molecule B7-1 and the human class II transactivator (CIITA)
proten. B7-1 provides an important second signal for T cell activation and CIITA
induces the expression of class II MHC molecules involved in antigen
presentation to T cells at high levels. As a result of keratinocyte B7-1 and
class II MHC expression, the mice are predisposed to develop substantially
increased contact hypersensitivity responses after sensitization to contact
This transgenic mouse line exhibits strongly enhanced skin immune response to
contact sensitizing chemicals without an appreciable change in the basal level
of cutaneous inflammation. In contrast, other transgenic mouse models showing
increased skin inflammation all have significant baseline changes in their
cutaneous biology subsequent to overespression of cytokines or growth