Cancer cell line derived from a relapsed pediatric patient with T-cell acute lymphoblastic leukemia.
- Well-characterized to have a point mutation of the p53 gene at codon 248.
- Expresses wild-type Bax and Bcl-xl, but does not express Bcl-2.
- Well used tool for p53 studies in leukemia.
p53 is a tumor suppressor protein that in humans is encoded by the TP53 gene. p53 regulates the cell cycle and thereby functions as a tumor suppressor that is involved in preventing cancer. Mutation or inactivation of the p53 gene has been implicated in a wide range of human malignancies, however most cases of acute lymphoblastic leukemia express normal p53. The MDM2 oncoprotein is the human homolog of the mouse double minute 2 (MDM2) gene product and acts as an important negative regulator of the p53 tumor suppressor by binding to the N-terminal trans-activation domain of p53 and inhibiting transcriptional activation of the p53 gene.
Emory researchers have established a cell line from a pediatric patient with T-cell acute lymphoblastic leukemia (ALL) with myeloid phenotype. This cell line has been characterized to contain a loss-of-function point mutation of the p53 gene at codon 248. The mutation has been shown to diminish the tumor-repression activity of p53 in the EU-7 cell line. This cell line provides a useful tool for studies of the role of p53 mutations in the pathogenesis of leukemia.
Cell line is available for out-license.
Zhou et al. 1995. Blood 85:1608-1614.
Findley et al. 1997. Blood 89:2986-2993.