Technology Listings

Spectroscopic Tags Using Dye-Embedded Nanoparticles and Surface-Enhanced Raman Scattering


Surface-enhanced Raman nanoparticles for multiplexed analysis of molecular biomarkers and multiparameter flow cytometry.

Technical Summary

Surface-enhanced Raman scattering (SERS) is capable of providing rich vibrational information at the level of single molecules and single nanoparticles. The inventors have developed a class of dye-embedded core-shell nanoparticles that are highly efficient for surface Raman enhancement and could be used as spectroscopic tags for multiplexed detection and spectroscopy. The core-shell particles contain a metallic core for optical enhancement, a reporter molecule for spectroscopic signature, and an encapsulating silica shell for protection and conjugation. The inventors have also incorporated isothiocyanate groups that can serve as molecular anchors for embedding organic dyes. Furthermore, the particles are stable in both aqueous electrolytes and organic solvents.

In comparison with fluorescent dyes and quantum dots, enhanced Raman probes contain a built-in mechanism for signal amplification and provide rich spectroscopic information under ambient experimental conditions. Unlike other biological labels, surface-enhanced Raman nanoparticles provide both high sensitivity and spectroscopic information.

Patent Information
App Type Country Serial No. Patent No. File Date Issued Date Expire Date
Utility(parent) United States 10/919,944 7,588,827 8/17/2004 9/15/2009 5/6/2026
Nationalized PCT - Foreign Japan 2006-524000 5244312 8/18/2004 4/12/2013 8/18/2024
Nationalized - Based on Foreign IP France 04820902.7 1660320 8/18/2004 5/29/2013 8/18/2024
Nationalized - Based on Foreign IP Germany 04820902.7 1660320 8/18/2004 5/29/2013 8/18/2024
Nationalized - Based on Foreign IP United Kingdom 04820902.7 1660320 8/18/2004 5/29/2013 8/18/2024
Nationalized PCT - Foreign Australia 2004308238 2004308238 2/21/2006   8/18/2024
Nationalized PCT - Foreign EP 04820902.7 1660320 3/8/2006 5/29/2013 8/18/2024
Nationalized PCT - Foreign Canada 2,536,266 2,536,266 10/5/2006 10/15/2013 8/18/2024
Tech ID: 03075
Published: 2/24/2009
Micro and Nanotechnology

Rajsekhar Guddneppanavar
Licensing Associate
Emory University

Shuming Nie
William Doering

Imaging Agents