Cell culture media that promotes the survival of human plasma cells and immunoglobin (Ig) secretion for laboratory research, diagnostic, and/or pharmaceutical reagents.
- Increases antibody-secreting cells (ASCs) found in blood after antigen exposure, which can be used to identify an active antigen or pathogen causing illness.
- Composed of mesenchymal stromal cells, increasing the survival potential in specific tissue culture.
- Allows the screening and identification of novel antibodies.
Cell culture is the foundation of biopharmaceutical development and manufacturing, and makes up a nearly $1.4 billion market in the U.S. alone. Media has many applications in research and pharmaceutical laboratories from facilitation of monoclonal antibodies to interrogating vaccine responses. Current practices limit the ability to isolate certain ASCs such as rare antigen-specific ASCs that represent less than 0.1% of available cells. Plasma cells isolated from blood, tissues, and secondary lymphoid organs readily die in less than 24 hours ex vivo and don’t survive in traditional cell culture conditions. In order to study vaccine responses, characterize myelomas, and study plasma cells in blood, cell culture conditions must be able to promote plasma cell survival.
Emory inventors have developed a special cell culturing media that promotes the survival of human plasma cells and Ig secretion. The specialized co-culturing method is composed of mesenchymal stromal cells, which have been shown to have a high proliferative potential in specific tissue culture. This in vitro culture method sustains human ASC 50 days or longer and allows for enough antibody secretion for a single plasma cell to be identified and isolated. The extended lifetime of the plasma cells has potential as a diagnostic reagent for interrogating vaccine efficacy, increasing Ig secretion to facilitate monoclonal antibodies more efficiently, and other applications. Researchers were able to pre-select ASC with desired antigen specificities, eliminating a step in current monoclonal antibody generation practices. Use of this culturing method enables the screening and production of novel antibodies for any antigen of interest.
Media has been utilized for patient samples from the Lowance Human Immunology Core and Pulmonary Division, Emory University.