Technology Listings


Recombinant Respiratory Syncytial Virus (RSV) Firefly Luciferase Reporter Strain

Application

Modified recombinant syncytial virus (RSV) strain expressing a bioluminescent reporter protein for high throughput screening and vaccine development.

Key Benefits
  • Provides a new high throughput screening tool for RSV vaccine development and drug discovery.
  • Firefly luciferase based assays show better signal-to-noise than fluorescence-based assays.
Market Summary

RSV is a major cause of respiratory illness, especially in young children and the elderly. In the United States, nearly all children have been infected with RSV by age two, resulting in 75,000-125,000 hospitalization and 4,000 deaths annually. Worldwide, the virus is responsible for 160,000 deaths per year. Currently, no vaccine or small molecule treatment for RSV exists, demonstrating a significant need for tools that allow for the development of potential treatments.

Technology Summary

Researchers at Emory have created a recombinant RSV strain that expresses firefly luciferase. Firefly luciferase is a bioluminescent protein from Photinus pyralis that is highly used as a genetic reporter in high throughput genetic screening assays. In the modified RSV, the bioluminescence serves as a reporter of RSV infection and replication activity. Therefore, this RSV strain serves as useful tool for high throughput screening of antiviral agents and measurement of anti-RSV antibodies.

Development Stage

The bioluminescent RSV strain has been engineered and is ready to distribute.

Patent Information
Tech ID: 14099
Published: 11/14/2016
Category
Research Tools
Vaccines

Contact
Cliff Michaels
Assistant Director
Emory University
404-727-3890
ccmicha@emory.edu

Inventor(s)
Richard Plemper
Martin Moore

Keywords
Immunology/Inflammation
Microbiology/Infectious Diseases
Respiratory