A quantitative enzyme-linked immunosorbent assay (ELISA) for monitoring endogenous rat tyrosine receptor kinase B (TrkB) activation.
- Two quantitative ELISA approaches provide reliable methods to quantify the intensity of TrkB receptor activation in primary neurons with endogenous TrkB receptor.
- Enables high-throughput drug screening targeted at the TrkB receptor.
Brain-derived neurotrophic factor (BDNF)/tyrosine receptor kinase B (TrkB) signaling plays essential roles in a variety of neurological processes. However, there is currently no quantitative ELISA approach to determine the mouse or rat TrkB receptor activity in primary neurons or central nervous system tissues.
Emory researchers have developed dyadic methods that take advantage of the protein sequence homology across different species. These methods allow for use in human, mouse, and rat species. These ELISA products can quantitatively examine BDNF-provoked TrkB activation in primary rat neurons and tightly correlate with p-TrkB immunoblotting results. The technology provides a powerful tool to examine endogenous TrkB receptor activation quantitatively in both primary neuronal cultures and brain tissues for basic and preclinical research needs.
The ELISA methods have been standardized and tightly correlate with p-TrkB immunoblotting results.