Technology Listings


Plasmids and Cell Lines Expressing Uncleavable Forms of Brain Angiogenesis Inhibitor 1 (BAI1) or its Extracellular Fragments

Application

Neovascular assays for the examination of antiangiogenic and antitumor effects of BAI1.

Technical Summary

The transmembrane protein BAI1 is subject to extracellular proteolytic cleavage, rendering a 120kDa fragment that is capable of inhibiting angiogenesis. Emory University researchers have generated several plasmids and cell lines capable of expressing multiple forms of this gene, making them useful for studying the therapeutic potential of these proteins.

An uncleavable form of BAI1 is available that was generated through site directed alanine mutagenesis of the G-protein coupled receptor (GPS) proteolytic cleavage site (S927→A927). This uncleavable form of BAI1 has been cloned into the constitutively active pcDNA3 vector, where it has been sequenced for verification and stably transfected into HEK 293 cells. The 120kDa cleavage product of BAI1 is not detectable in conditioned media taken from transformants. Both the pcDNA3::BAI1A927 construct and HEK 293 cells transfected with this plasmid are available.

Plasmids and cell lines containing only the extracellular region of BAI1 are also available. The extracellular domain of BAI1 (a.a.1-929; truncated at GPS cleavage site) was cloned into pcDNA3 (pcDNA3::BAI1-ECD) and pTRE2 (pTRE2::BAI1-ECD) to drive constitutive and tetracycline driven expression, respectively. These vectors contain myc and His tags. Both pcDNA3::BAI1-ECD and pTRE2::BAI1-ECD are available in several different cell lines:

Publications

Cancer Res. 2009 69(3): 1212
Am J Pathol. 2003 January; 162(1): 19-27.
Oncogene. 2005 May 19;24(22):3632-3642.

Patent Information
Tech ID: 10018
Published: 4/15/2010
Category
Research Tools

Contact
Justin Burns
Licensing Associate
Emory University
justin.burns@emory.edu

Inventor(s)
Erwin Van Meir

Keywords
Neuroscience/Pain
Assay
Cell Line