- Western Blot
Human, mouse and rat SRPK2
The T492 antibody was generated using acetyl-DRSR[pT]VSASSTG-Ahx-C-amide as the antigen.
150-200 mL at 1.5-3 mg/mL
SRPK, a family of cell cycle regulated protein kinases, phosphorylate Serine/Arginine (SR) domain-containing proteins in nuclear speckles and mediate the pre-mRNA splicing. SRPK2 is highly expressed in brain, and Dr. Ye's group has shown that SRPK2 binds and phosphorylates acinus, an SR protein essential for RNA splicing, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not A2 upregulation. Ablation of SRPK2 abrogates cyclin A1 expression in leukemia cells and arrest cells at G1 phase, while SRPK2 overexpression increases leukemia cell proliferation. Further, SRPK2 is highly expressed in some of human AML patients and correlate with elevated cyclin A1 expression levels, fitting with the oncogenic activity of cyclin A1 in leukemia.
Publication: Jang et al. J Biol Chem. 284(36):24512-25. (2009)