Technology Listings


Phospho-MSTI T387 Antibody

Applications
  • Western Blot
  • Immunoprecipitation
  • Immunohistochemistry

Specificity

Human, mouse and rat

Subtype

Rabbit Polyclonal

Antibody Synthesis

The T387 antibody was generated using EEGTMKRRDEp-TMQPA as the antigen.

Amount Available

150-200 mL at 1.5-3 mg/mL

Technical Summary

Oxidative stress can induce apoptosis through activation of MstI, subsequent phosphorylation of FOXO and nuclear translocation. MstI is a common component of apoptosis initiated by various stresses. MstI kinase activation requires autophosphorylation and proteolytic degradation by caspases. Dr. Ye's group has demonstrated that MstI is a physiological substrate of Akt. Akt phosphorylation of MstI diminishes its apoptotic cleavage by caspases and prevents its kinase activity on FOXO3. MstI directly binds to Akt, which is regulated Akt kinase activity. Akt phosphorylates MstI on the Thr387 residue and protects MstI from apoptotic cleavage in vitro and in apoptotic cells. Furthermore, Akt phosphorylation of MstI strongly inhibits its kinase activity on FOXO3. The findings support that Akt blocks MstI-triggered FOXO3 nuclear translocation by phosphorylating MstI, promoting cell survival.

Publication: Jang et.al., (2007).  J. Biol. Chem. 282(42):30836-44.

Patent Information
Tech ID: 09071
Published: 4/29/2009
Category
Research Tools

Contact
Cliff Michaels
Assistant Director
Emory University
404-727-3890
ccmicha@emory.edu

Inventor(s)
Keqiang Ye

Keywords
Antibody