- Western Blot
Human, mouse and rat
The T219 antibody was generated using TRRG-T(PO3H2)-RGGARGRGRGC-NH2 as the antigen.
150-200 mL at 1.5-3 mg/mL
Nuclear PI3K and its downstream effectors play essential roles in a variety of cellular activities including cell proliferation, survival, differentiation, and pre-mRNA splicing. Aly is a nuclear speckle protein implicated in mRNA export. Dr. Ye's group has shown that Aly is a physiological target of nuclear PI3K signaling, which regulates its subnuclear residency, cell proliferation, and mRNA export activities through nuclear Akt phosphorylation and phosphoinositide association. Nuclear Akt phosphorylates Aly on threonine-219, which is required for its interaction with Akt. Aly binds phosphoinositides, and this action is regulated by Akt-mediated phosphorylation. Phosphoinositide binding but not Akt phosphorylation dictates Aly's nuclear speckle residency. Depletion of Aly results in cell growth suppression and mRNA export reduction. As a result, nuclear PI3K signaling mediates both cell proliferation and mRNA export functions of Aly.
Publication: Masashi Okada, et al. (2008). Proc. Natl. Acad. Sci. USA. 105 (25): 8649-8654.