- Western blot
- Flow cytometry
Antibody targeting human CD47.
CD47 is a cell surface glycoprotein that was originally defined as a tumor-specific marker for ovarian carcinoma but it is now known to be expressed on all hematopoietic cells and most other cell types. CD47 plays an important role in modulating neutrolphil (PMN) migration across endothelial and epithelial monolayers. In addition, it has been broadly implicated in multiple cellular processes including neutrophil phagocytosis, T cell activation, T and B cell apoptosis, platelet activation, and stroma supported erythropoiesis.
PF3.1 anti-CD47 monoclonal antibody was raised by immunizing rabbits to a corresponding region in the C-terminal domain of CD47. This antibody has shown to be very effective and specific in research tools such as flow cytometry, immunofluorescence, and western blot.