Multifunctional Magnetic Nanoparticle Probes for Molecular Imaging
- Sensitive, specific, and rapid nucleic acid detection.
- High signal-to-noise ratio and ultra-low false-positives in molecular imaging.
- Detection of gene expression, including genetic expression associated with an infection or disease state.
This invention provides for multifunctional magnetic nanoparticle probe for molecular imaging and monitoring, comprising a nucleic acid or polypeptide probe, a delivery ligand, and a magnetic nanoparticle with a biocompatible coating. The probe can include a fluorescent or luminescent resonance energy transfer moiety. The researchers have designed the nucleic acid or polypeptide probes to bind to a target and generate an interaction observable with magnetic resonance imaging (MRI) or optical imaging with improved spatial resolution and versatility. The invention thereby provides detectable signals for rapid, highly specific, and sensitive detection of nucleic acids, polypeptides, and interactions thereof in vivo with enhanced signal-to-noise ratio. These innovations and their integration will provide a platform for a new generation of biomagnetic contrast agents for deep tissue molecular imaging and monitoring. The multifunctional magnetic nanoprobes offer a novel transduction mechanism for the in vivo detection of molecular markers for disease, infection, and injury.