Recombinant viral system created to study Gammaherpesvirus pathogenesis.
Neoplastic disease is a frequent complication in patients with immunodeficiency diseases (i.e. AIDS). Many of these neoplasms are caused by either Epstein-Barr virus (EBV) or Kaposi's sarcoma-associated herpes virus (KSHV). The treatment of these immunosuppressed patients can be challenging. The viral origin of these tumors offer targets for the development pathogenesis-based therapies. Gammaherpesvirus 68 (MHV68) infection in mice is a model system for studying these tumors, enabling evaluation of virus-host interactions that influence disease.
Emory Investigators generated a recombinant MHV68 virus that encodes an antibiotic (hygromycin) resistance protein fused to enhanced green fluorescent protein as a means to select cells in culture that harbor latent virus. With this tool, MHV68 pathogenesis and potentially the pathogenesis of neoplastic disease can be further explored.
Publication: Forrest JC, Speck SH. J Virol. 2008; 82:7688-7699.