- Western Blot
Human, mouse and rat
Synthesized S315 phosphorylated peptide (CRRRKAD-(PO3)S-LEVQQMKAQAREEKARK-COOH) was conjugated to keyhole limpet hemocyanin (KLH) and injected into rabbits. Antibodies were affinity purified from serum using unphosphorylated peptide crosslinked to Affi-Gel 10 or Affi-Gel 15.
150-200 mL at 1.5-3 mg/mL
The neurofibromatosis-2 (NF2) tumour-suppressor gene encodes an intracellular membrane-associated protein, called merlin, whose growth-suppressive function is dependent on its ability to form interactions through its intramolecular amino-terminal domain (NTD) and carboxy-terminal domain (CTD). Merlin phosphorylation plays a critical part in dictating merlin NTD/CTD interactions as well as in controlling binding to its effector proteins. Dr. Ye's group demonstrated that the protein kinase Akt directly binds to and phosphorylates merlin on residues Thr 230 and Ser 315, which abolishes merlin NTD/CTD interactions and binding to merlin's effector protein PIKE-L and other binding partners. These studies demonstrate that Akt-mediated merlin phosphorylation regulates the function of merlin in the absence of an inactivating mutation.
Publication: Tang et. al., (2007). Nature Cell Biol. 9(10):1199-1207.