Immortalized (using polyoma middle T-antigen) mouse aortic endothelial primary cell lines for studying vascular biology and pathophysiology, including cardiovascular research.
- Immortalized from mouse artery endothelial cells using polyoma middle T-antigen.
- Validated retention of primary endothelial markers and structural phenotype in cell culture.
- Wild-type, p47phox-/-, eNOS-/-, and caveolin-1-/- knockout aortic endothelial cell llines.
A lack of primary mouse aortic endothelial cells for cell culture remains a hindrance in endothelial cell biology research. One major challenge is developing endothelial cells for cell culture that retain their original endothelial phenotype. The inventors created a series of immortalized mouse aortic endothelial WT and genetically modified cell lines that retain their endothelial cell markers (PECAM1, eNOS, VE-cadherin, and von Willebrand Factor) and the ability to responds to physiological stiumuli such as shear stress and TNFÎ± and form endothelial tube structure.
Cell lines are available for licensing.
Publication: Ni, C .et al. (2014). Vascular Cell. 6:7