Technology Listings


Hydroxyl-Derivatized Surface Coatings for Minimizing Nonspecific Binding of Nanoparticles

Application

Surface coatings for minimizing nonspecific binding of quantum dots and other nanoparticles.

Key Benefits

Coating method generated a 140-fold reduction in nonspecific binding relative to that of carboxylated quantum dots and 10-20-fold reduction relative to that of PEG- and protein-coated quantum dots.

Technical Summary

Quantum-dot (QD) nanocrystals are promising fluorescent probes for multiplexed staining assays in biological applications. However, nonspecific QD binding to cellular membranes and proteins remains a limiting factor in detection sensitivity and specificity. Drs. Shuming Nie and Brad Kairdolf have developed a new class of hydroxyl (-OH)-coated QDs for minimizing nonspecific cellular binding and for overcoming the bulky size problems encountered with previous surface coatings. The hydroxylated QDs are prepared from carboxylated (-COOH) dots via a hydroxylation and cross-linking process. With a compact hydrodynamic size of 13-14 nm (diameter), the quantum dots are highly fluorescent (>60% quantum yields) and stable under both basic and acidic conditions. By using human cancer cells, the researchers compared the nonspecific binding properties against that of the industry standards: carboxylated, protein-coated, and poly(ethylene glycol) (PEG)-coated QDs. Quantitative cellular staining data indicate that the hydroxylated QDs result in a dramatic 140-fold reduction in nonspecific binding relative to that of carboxylated dots and a significant 10-20-fold reduction relative to that of PEG- and protein-coated dots.

Potential Market

With minimized nonspecific binding and background interference, QD probes are especially well-suited for analyzing cancer biomarkers that are present at low concentrations or in small numbers of cells. The cancer biomarker market comprises 55% of the entire biomarker market with revenues in excess of $3 billion annually.

Publication: Anal Chem. 2008 Apr 15;80(8):3029-34.

Patent Information
App Type Country Serial No. Patent No. File Date Issued Date Expire Date
PCT PCT PCT/US2009/033196   2/5/2009   8/8/2010
Continuation United States 13/916,937 2/9/2009    
Tech ID: 08028
Published: 9/22/2010
Category
Micro and Nanotechnology
Diagnostics

Contact
Rajsekhar Guddneppanavar
Licensing Associate
Emory University
RGUDDNE@emory.edu

Inventor(s)
Brad Kairdolf
Shuming Nie

Keywords
Biomarker
Oncology