Human lung microvascular cell line (HuLEC-1).
Emory and CDC researchers have isolated human lung microvascular cells (HuLEC) and derived the first immortalized human microvascular endothelial cell line of dermal origin. The immortalized cell line (HuLEC-1) maintains microvascular endothelial cell characteristics, including formation of tubules in vitro and the expression of low density lipoprotein (LDL) and von Willebrand’s Factor. These cells show endogenous expression of vascular cell adhesion molecule, expression of E-selectin following TNF-alpha stimulation, and adherence of M. tuberculosis to the cell line. This cell line can be used to study the role of this molecule in a variety of pathological conditions, including allograft rejection, atherosclerosis, primary pulmonary hypertension, and various inflammatory and infectious disorders of the lung and other organs.
Publication: Ades EW, et al. (1992) J. Invest. Dermatol. 99:683-690.