Technology Listings


Human Glioma Cell Lines for Doxyxycline-Inducible Gene Expression

Application

Multiple glioma cell lines containing a doxycycline-inducible system for gene expression.

Technical Summary

In an effort to better understand the temporal role of tumor suppressors in glioma formation, Emory University researchers have generated a number of different human glioma cell lines that contain a doxycycline system for inducible gene expression. The cell lines chosen are ideal for study as they possess heterogeneous backgrounds for PTEN, BAI1, p53, p14ARF, and p16. Tet-on lines were generated by stably transfecting cells with the rtTA expression vector, while tet-off lines were generated by stably transfecting the tTA expression vector. In all instances, tetracycline-responsive clones were selected, and transiently transfected with tetracycline-responsive expression constructs to ensure stable integration. Several of these lines have been implanted into nude mice, where they exhibit significant tumorgenicity, and display appropriate doxycycline responses.

Publications

Kaur B, et al. Oncogene. 2005: 24(22): 3632-42.
Kaur B, et al. Cancer Res. 2009; 69(3): 1212-20.

Patent Information
Tech ID: 10021
Published: 3/23/2010
Category
Research Tools

Contact
Justin Burns
Licensing Associate
Emory University
justin.burns@emory.edu

Inventor(s)
Erwin Van Meir

Keywords
Cell Line
Neuroscience/Pain