Multiple glioma cell lines containing a doxycycline-inducible system for gene expression.
In an effort to better understand the temporal role of tumor suppressors in glioma formation, Emory University researchers have generated a number of different human glioma cell lines that contain a doxycycline system for inducible gene expression. The cell lines chosen are ideal for study as they possess heterogeneous backgrounds for PTEN, BAI1, p53, p14ARF, and p16. Tet-on lines were generated by stably transfecting cells with the rtTA expression vector, while tet-off lines were generated by stably transfecting the tTA expression vector. In all instances, tetracycline-responsive clones were selected, and transiently transfected with tetracycline-responsive expression constructs to ensure stable integration. Several of these lines have been implanted into nude mice, where they exhibit significant tumorgenicity, and display appropriate doxycycline responses.
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