- Western Blot
Mouse and human Dnmt3a - Specifically K44me2 of mDnmt3a and K47me2 of hDNMT3A
MPP8 mediates the interactions between DNA methyltransferase Dnmt3a and H3K9 methyltransferase GLP/G9a. DNA methylation and H3K9 methylation appear to co-occur in chromatin. The antibody was raised against the peptide CSATAR(Kme2)VGRPGR for mouse and YEPSTTAR(Kme2)VGRPGR for human. The peptide was conjugated to KLH via the N-terminal cysteine.
Publication: Chang, Y et al. Nat Commun, 2011. 2: 533.