- Flow Cytometry
- Functional Inhibition
Transepithelial migration of neutrophils (PMN) is a defining characteristic of active inflammatory states of mucosal surfaces. Little is known about the molecular interactions between PMN and columnar epithelia during transepithelial migration. Emory researchers have developed a monoclonal antibody, C5/D5 IgG, raised against integrin associated proteins (CD47). The antibody is capable of inhibiting PMN migration across polarized monolayers of the human intestinal epithelial cell line T84 in a bidirectional fashion.
Publication: Parkos, C. A., S. P. Colgan, et al. (1996). The Journal of Cell Biology 132(3): 437-450.