Technology Listings


Antibody for Detection of Mouse ENaC

Application

Polyclonal antibody for the detection of mouse Epithelial Sodium Channel (ENaC) a subunit in western blots.

Key Benefits
  • Affinity purified rabbit polyclonal antibody recognizes the 85kDa a subunit of mouse Epithelial Sodium Channel (ENaC).
  • Immunizing peptide consisting of mouse specific sequence was used to limit cross-reactivity.
Technical Summary

Polarized epithelium bears many responsibilities including maintenance of apical and basal fluid homeostasis. These cells affect important fluid characteristics by moving a range of ions both into and out of the extracellular space using ion-specific channels, transporters, and exchangers. One such channel is the Epithelial Sodium Channel ENaC, an amiloride-sensitive heterotrimeric protein complex that contributes to sodium reabsorption in a number of major organs including the lungs, kidney, and colon. In addition to fluid regulation, ENaC has been implicated in a host of functions including taste and blood pressure maintenance, and its dysregulation can cause health complications that include severe arterial hypertension (Liddel's Syndrome) and pseudohypoaldosteronism. To facilitate further study of ENaC in a rodent model system, a rabbit polyclonal antibody has been raised to the a subunit of mouse ENaC.

Patent Information
Tech ID: 08018
Published: 2/24/2009
Category
Research Tools

Contact
Cliff Michaels
Assistant Director
Emory University
404-727-3890
ccmicha@emory.edu

Inventor(s)
Young Hee Kim
Seongun Hong
Susan Wall
James Melvin

Keywords
Antibody