Technology Listings


Antibody against Phosphorylated Ser794 of Ataxia-Telangiectasia Mutated (ATM)

Applications
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
Specificity

Human

Immunogen

KLH tagged synthetic 16mer phosphopeptide corresponding to residues surrounding Ser794 of human ATM (Acetyl-CLSNATKpSPNKIASG-amide).

Subtype

Rabbit polyclonal

Technical Summary

Ataxia-Telangiectasia Mutated (ATM) is an important kinase that plays key roles in mediated cellular responses to DNA damage. Mutations of ATM can lead to abnormalities in immune function as well as sensitivity to radiation, predisposition to cancer, accelerated aging and neurodegeneration. How ATM is activated following DNA damage is not clear. Dr. Mao's group at Emory have recently identified that the ATM protein gets activated when it is phosphorylated at the Ser794 position by Cyclin Dependent Kinase 5 (Cdk5). Further, they have determined that monitoring the phosphorylation at Ser794 can be regarded as a direct measure of ATM function, which is critical to determine in physiological or pathological settings.

This antibody can be used in a variety of research, clinical and diagnostic settings. Some examples of its uses are (a) monitoring the effectiveness of radiation therapy or chemotherapy (b) detecting DNA damaging agents in a biological or environmental sample (c) monitoring interactions of ATM with other proteins via the use of ATM peptide sequence containing Ser794 (d) cell-based or cell-free assays for identifying agents that modulate ATM activity and / or its interaction with other proteins by using the Ser794 phosphorylation state as the read-out.

Publication: Tian, B et al. Nat Cell Biol. 2009 Feb;11(2):211-8.

Patent Information
Tech ID: 08038
Published: 4/27/2011
Category
Research Tools

Contact
Cliff Michaels
Assistant Director
Emory University
404-727-3890
ccmicha@emory.edu

Inventor(s)
Zixu Mao

Keywords
Neuroscience/Pain
Oncology
Antibody