- Western Blot
Calcium is responsible for regulating various cellular processes. The elevation of intracellular calcium regulates the activity of Na+/H+ exchanger 3 (NHE3). Emory inventors have identified a polyclonal antibody which cross reacts with inositol triphosphate (IP3) receptor-binding protein (IRBIT). IRBIT is released with IP3, binds NHE3 and activates NHE3 activity in response to calcium. The interaction of IRBIT is necessary for the stimulation of NHE3 activity.
Publication: He, P., et al. (2008). J. Biol. Chem. 283 (48):33544-33553