- Western Blot
Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by extracellular senile plaque deposits, composed of amyloid beta and intracellular Neurofibrillary tangles (NFT), made of truncated and hyperphosphorylated tau.Â Researchers at Emory have shown that tau is cleaved by asparagine endopeptidase (AEP) resulting in a truncated tau (N279 tau) associated with NFT in both human tissue samples and in rodent AD models. N279 tau can be detected prior to cognitive defects in the tau P301S-knock in mouse model of AD suggesting AEP cleavage of tau could be a biomarker for early stages of AD. Emory researchers have developed a rabbit monoclonal antibody that selectively recognizes the AEP cleaved type of Tau found in AD pathology but not full-length Tau. This antibody has potential uses in western blotting, flow cytometry and ELISA.
Publication: Zhang Z, et al. Nat Med. 2014 Nov;20(11):1254-62.