Technology Listings


Antibody Raised Against ERK302me1 (Human Estrogen Receptor Alpha Monomethylated at Lysine Residue 302)

Applications
  • Western Blot
  • Elisa
Specificity

Human

Subtype

Polyclonal

Immunogen

A peptide corresponding to amino acids 297-308 of ER mono-methylated at K302 (NH2-MIKRS(meK)KNSLALCCOOH)

Technical Summary

Estrogen receptor alpha (ER) is a ligand-dependent transcription factor. Upon binding estrogen, ER recruits coactivator complexes with histone acetyltransferase or methyltransferase activities to activate downstream target genes. In addition to histones, coactivators can modify ER itself and other proteins in the transactivation complex. Here, we show that ER is directly methylated at lysine 302 (K302) by the SET7 methyltransferase. SET7-mediated methylation stabilizes ER and is necessary for the efficient recruitment of ER to its target genes, and their transactivation. The SET7-ER complex structure reveals the molecular basis for ER peptide recognition and predicts that modifications or mutations of nearby residues would affect K302 methylation. Indeed, a breast cancer-associated mutation at K303 (K303R) alters methylation at K302 in vitro and in vivo. These findings raise the possibility that generation, recognition, and removal of modifications within the ER hinge region generates “ER modification cassettes†that yield distinct patterns for signaling downstream events.

Publication: Subramanian K, et al. Mol Cell. 2008;30:336-347.

Patent Information
Tech ID: 10187
Published: 9/14/2011
Category
Research Tools

Contact
Justin Burns
Licensing Associate
Emory University
justin.burns@emory.edu

Inventor(s)
Paula Vertino

Keywords
Antibody