- Western Blot
Alzheimer’s disease is the most common form of severe dementia; yet, there is no cure. Although it is known that Amyloid b (Ab) plaques contribute to the disease, additional research tools are necessary to determine how these plaques form. Researchers at Emory have found that a lysosomal protease called asparagine endopeptidase (AEP) can cleave amyloid precursor protein (APP). When amyloid precursor protein (APP) is cleaved, the resulting peptides contribute to Amyloid b (Ab) plaque formation and the progression of Alzheimer’s disease. Dr. Ye’s lab characterized two novel sites of APP cleavage by the AEP enzyme (APP sites N373 and N585). They then generated antibodies that, unlike what is currently available, specifically detect the APP peptides that result from AEP cleavage. The antibodies specifically recognize the following regions: APP amino acids 1-373, APP amino acids 1-585, and APP amino acids 586-695, but not the full length).
Publication: Zhang, Z. et al. -Secretase cleaves amyloid precursor protein and regulates the pathogenesis in Alzheimer's disease. Nat. Commun. 2015; 6: 8762