- Western blot
Recombinant His-tagged bovine polyadenylate-binding nuclear protein 1 (PABPN1)
The bovine PABPN1 coding sequence was inserted into an Escherichia coli vector and was used for the production of His-tagged PABPN1. This PABPN1 sequence was then purified and used for the immunization of rabbits to obtain polyclonal anti-PABPN1 antibody.
Apponi et al. Hum. Mol. Genet. 2010. 19:1058-1065.
Kuhn et al. J. Biol. Chem. 2003. 278:16916-16925.