Technology Listings


A High Throughput Assay to Screen for BMP Modulators

Application

A high throughput screening assay to identify bone morphogenic protein (BMP) modulators.

Key Benefits
  • Homogeneous proximity-induced interaction method.
  • Robust, highly sensitive, easy to use method of monitoring BMP interaction.
  • Characterization of potential BMP modulators for therapeutic development.
Technical Summary

The BMP/BMP receptor interaction represents an important target for the discovery of novel therapeutic agents for the treatment of various BMP-signaling mediated diseases. Emory researchers have shown that BMP is a potent pro-inflammatory cytokine that causes hypertension and atherosclerosis in mice. In addition, they have demonstrated that these BMP-induced conditions could be completely prevented by treating mice with the BMP inhibitor noggin. In order to discover noggin-like small molecule inhibitors, a homogeneous proximity-induced interaction method to monitor the association of BMP and its receptor has been developed. A BMP/BMP receptor based fluorescence resonance energy transfer (FRET) technology has been developed that is highly sensitive and has a high density plate format. The FRET signal can be inhibited by noggin in a dose-dependent manner, which validates this assay for the identification of BMP modulators. The assay features a one-step "mix-and-read" method and is particularly suited for use in high throughput screening or ultra high throughput screening for BMP modulators.

Developmental Stage & Potential Market
  • This technology has been reduced to practice and has successfully identified four highly active, potential BMP inhibitors.
  • Due to the broad spectrum of activity that BMP inhibitors exhibit, a technology that is able to effectively identify potential inhibitors is needed.
Patent Information
Tech ID: 07107
Published: 2/24/2009
Category
Research Tools
Drug Discovery

Contact
Cliff Michaels
Assistant Director
Emory University
404-727-3890
ccmicha@emory.edu

Inventor(s)
Kyung Hwa Chang
Yuhong Du
Haian Fu
Hanjoong Jo

Keywords
Cardiovascular
Musculoskeletal
Assay